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4 × 44k human genome cgh microarray kit (for derivative chromosomes 6p and 9q)  (Agilent technologies)


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    Agilent technologies 4 × 44k human genome cgh microarray kit (for derivative chromosomes 6p and 9q)
    4 × 44k Human Genome Cgh Microarray Kit (For Derivative Chromosomes 6p And 9q), supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/4+%C3%97+44k+human+genome+cgh+microarray+kit+(for+derivative+chromosomes+6p+and+9q)/pmc03157243-81-6-28
    Average 90 stars, based on 1 article reviews
    4 × 44k human genome cgh microarray kit (for derivative chromosomes 6p and 9q) - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Priority Report: Rearrangements and Amplification of IER3 (IEX-1) Represent A Novel And Recurrent Molecular Abnormality In Myelodysplastic Syndromes (MDS)
    Article Snippet: Southern blotting In order to determine whether the rearrangement in the propositus was a simple translocation of IER3 or more complex, DNA extracted from der(6) and der(9) somatic hybrid cells, propositus-derived whole marrow cells, and control marrow cells was digested with BamHI and EcoRI restriction endonucleases (New England Biolabs, Cambridge, MA), subject to agar gel electrophoresis for 24 hrs, blotted overnight on a nylon membrane, crosslinked with ultraviolet radiation, hybridized with a radiolabeled IER3 probe and salmon sperm blocking DNA, washed, and visualized by autoradiography.

    Hybridization:

    Article Title: Priority Report: Rearrangements and Amplification of IER3 (IEX-1) Represent A Novel And Recurrent Molecular Abnormality In Myelodysplastic Syndromes (MDS)
    Article Snippet: .. Array-based comparative genomic hybridization (aCGH) High-resolution aCGH was performed with a custom 4 × 44K Human Genome CGH Microarray Kit (for derivative chromosomes 6p and 9q) and an off-the-shelf 244A kit (whole genome) (Agilent Technologies, Santa Clara, CA), following the protocol suggested by the manufacturer. .. Briefly, 800 ng of sample and control DNA, amplified from microdissected chromosomes using degenerate oligonucleotide primers, were labeled with Cy5 or Cy3 (BioPrime Array CGH Genomic Labeling Module, Invitrogen) and purified using Vivaspin 500 columns (Sartorius Stedim Biotech, Aubagne, France).

    Microarray:

    Article Title: Priority Report: Rearrangements and Amplification of IER3 (IEX-1) Represent A Novel And Recurrent Molecular Abnormality In Myelodysplastic Syndromes (MDS)
    Article Snippet: .. Array-based comparative genomic hybridization (aCGH) High-resolution aCGH was performed with a custom 4 × 44K Human Genome CGH Microarray Kit (for derivative chromosomes 6p and 9q) and an off-the-shelf 244A kit (whole genome) (Agilent Technologies, Santa Clara, CA), following the protocol suggested by the manufacturer. .. Briefly, 800 ng of sample and control DNA, amplified from microdissected chromosomes using degenerate oligonucleotide primers, were labeled with Cy5 or Cy3 (BioPrime Array CGH Genomic Labeling Module, Invitrogen) and purified using Vivaspin 500 columns (Sartorius Stedim Biotech, Aubagne, France).



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